Other publications from Group members

@article{0e,
title = {Mitogen-activated protein kinase phosphatase 1 (MKP1) negatively regulates the production of Reactive Oxygen Species during Arabidopsis immune responses},
author = {Viviana Escudero and Miguel Ángel Torres and Magdalena Delgado and Sara Sopeña-Torres and Sanjay Swami and Jorge Morales and Antonio Muñoz-Barrios and Hugo Mélida and Alan M. Jones and Lucía Jordá and Antonio Molina},
doi = {10.1094/MPMI-08-18-0217-FI},
issn = {0894-0282},
year = {2019},
date = {2019-04-27},
urldate = {2018-11-02},
journal = {Mol Plant Microbe Interact.},
volume = {32},
number = {4},
abstract = {Genetic ablation of the β-subunit of the heterotrimeric G protein complex in agb1-2 confers defective activation of Molecular-Associated Molecular Pattern (MAMP)-triggered immunity, resulting in agb1-2 enhanced susceptibility to pathogens, like the fungus Plectosphaerella cucumerina BMM (PcBMM). A mutant screen for suppressors of agb1-2 susceptibility (sgb) to PcBMM identified sgb10, a new null allele ( mkp1-2) of the mitogen-activated protein kinase phosphatase 1 (MKP1). The enhanced susceptibility of agb1-2 to the bacterium Pseudomonas syringae pv. tomato DC3000 and the oomycete Hyaloperonospora arabidopsidis is also abrogated by mkp1-2. MKP1 negatively balances production of reactive oxygen species (ROS) triggered by MAMPs since ROS levels are enhanced in mkp1. The transcriptional expression of RBOHD, encoding a NADPH oxidase producing ROS, is upregulated in mkp1 plants upon MAMPs treatment or pathogen infection. Moreover, MKP1 negatively regulates RBOHD activity because ROS levels upon MAMP treatment are increased in mkp1 plants constitutively overexpressing RBOHD(35S::RBOHD mkp1). A significant reprograming of mkp1 metabolic profile occurs with more than 170 metabolites, including antimicrobial compounds, showing differential accumulation in comparison to wild-type plants. These results suggest that MKP1 functions downstream of the heterotrimeric G protein during MAMP-triggered immunity, directly regulating the activity of RBOHD and ROS production, and other immune responses.},
howpublished = {[Epub ahead of print]},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

@article{0d,
title = {YODA MAP3K kinase regulates plant immune responses conferring broad-spectrum disease resistance},
author = {Sara Sopeña-Torres and Lucía Jordá and Clara Sánchez-Rodríguez and Eva Miedes and Viviana Escudero and Sanjay Swami and Gemma López and Mariola Piślewska-Bednarek and Ines Lassowskat and Justin Lee and Yangnan Gu and Sabine Haigis and Danny Alexander and Sivakumar Pattathil and Antonio Muñoz-Barrios and Pawel Bednarek and Shauna Somerville and Paul Schulze-Lefert and Michael G Hahn and Dierk Scheel and Antonio Molina},
doi = {10.1111/nph.15007},
year = {2018},
date = {2018-04-10},
urldate = {2018-02-16},
journal = {New Phytol.},
volume = {218},
number = {2},
pages = {661-680},
abstract = {Mitogen-activated protein kinases (MAPKs) cascades play essential roles in plants by transducing developmental cues and environmental signals into cellular responses. Among the latter are microbe-associated molecular patterns perceived by pattern recognition receptors (PRRs), which trigger immunity. We found that YODA (YDA) - a MAPK kinase kinase regulating several Arabidopsis developmental processes, like stomatal patterning - also modulates immune responses. Resistance to pathogens is compromised in yda alleles, whereas plants expressing the constitutively active YDA (CA-YDA) protein show broad-spectrum resistance to fungi, bacteria, and oomycetes with different colonization modes. YDA functions in the same pathway as ERECTA (ER) Receptor-Like Kinase, regulating both immunity and stomatal patterning. ER-YDA-mediated immune responses act in parallel to canonical disease resistance pathways regulated by phytohormones and PRRs. CA-YDA plants exhibit altered cell-wall integrity and constitutively express defense-associated genes, including some encoding putative small secreted peptides and PRRs whose impairment resulted in enhanced susceptibility phenotypes. CA-YDA plants show strong reprogramming of their phosphoproteome, which contains protein targets distinct from described MAPKs substrates. Our results suggest that, in addition to stomata development, the ER-YDA pathway regulates an immune surveillance system conferring broad-spectrum disease resistance that is distinct from the canonical pathways mediated by described PRRs and defense hormones.

Keywords: ERECTA, cell wall, mitogen-activated protein kinases (MAPKs), pattern recognition receptor (PRR), plant immunity, receptor-like kinase (RLK), stomata},
keywords = {},
pubstate = {published},
tppubtype = {article}
}